Sybr Green Qpcr Protocol Bio Rad

Sybr green supermixes for real time pcr.

Sybr green qpcr protocol bio rad.

Any chemistry supermixes for sybr green or probe based detection chemistry. Any instrument universal reference dye is compatible with all qpcr platforms. Forward and reverse primers diluted to working concentration 10µm working stocks are sufficient for most assays. General description kicqstart sybr green qpcr readymix is a 2x concentrated ready to use reaction cocktail that contains all components except primers and template for real time quantitative pcr qpcr this unique combination of proprietary buffer stabilizers and hot start taq dna polymerase delivers maximum pcr efficiency sensitivity specificity and robust fluorescent signal using fast.

Our real time pcr supermixes are designed for. Any conditions our patented sso7d fusion polymerase guarantees superior qpcr performance under. Ssoadvanced universal sybr green supermix is an exclusive high performance real time pcr reagent based on bio rad s patented sso7d fusion protein polymerase technology and advanced buffer formulation. This is the only sybr green mix we use with our cfx connect and we came across it via bio rad s awesome service contract.

This supermix is uniquely formulated to provide higher processivity increased pcr inhibitor tolerance and robust performance with challenging templates and targets in sybr green based qpcr. Itaq universal sybr green supermix provides reliable gene expression data. Standard curve r 2 0 999 β actin efficiency 92 6 slope. We often run qpcr on smaller populations of sorted cells and i m sometimes a bit iffy on the amount of rna.

Available with our proprietary passive reference dyes our sybr green pcr supermixes feature advanced enzymes and buffer formulations to ensure superior speed sensitivity and. Qpcr sybr green mix refer to qpcr selection guides part 1 and part 2 dna cdna template cdna reaction diluted 1 10 to detect a medium to highly expressed targets or 1 2 to 1 5 for rare transcripts or 10 ng to 100 ng gdna. As the pcr reaction proceeds at each round of amplification sybr green dye binds to dsdna as it polymerizes resulting in an increase in the level of fluorescence at the end of each extension step. Itaq universal sybr green supermix produced greater than 90 efficiency over 6 orders of linear dynamic range.

Bio rad s supermixes can make any qpcr experiment easier faster and more effective. Sybr green master mix advantages. The human β actin gene was amplified from hela cdna 100 ng 100 fg using a cfx96 real time pcr detection system. Bio rad s sybr green supermixes for real time pcr are proven formulations for a wide variety of qpcr assays offering reliable consistent real time pcr results.

Sybr green dye is a fluorescent double stranded dna dsdna binding dye that is used to track the progress of dna amplification in real time pcr experiments. When making the request be sure to include the lot number and catalog number listed on the iq sybr green supermix label. Sybr green qpcr supermixes are compatible with most protocols for qpcr. I don t have a whole lot to compare it to but it works phenomenally in our hands and i would be very hesitant to trust another green mix with my precious rna samples.