Powertrack sybr green master mix other master mix qpcr master mix 2x 260 µl 260 µl forward primer 10 µm 26 µl see manufacturer s reverse primer 10 µm 26 µl recommendation nuclease free water 65 µl to bring to 377 µl bring volume to 377 µl amount shown is for a final primer concentration of 500 nm. The mix is optimized for sybr green re actions and contains sybr green i dye amplitaq gold dna polymerase dntps with dutp passive reference and optimized buffer components. Fast sybr green master mix contains everything you need excluding the template and primers in a convenient 2x mix.
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